User:Karlena L. Brown/Notebook/PVOH Research/2013/04/05: Difference between revisions

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(Autocreate 2013/04/05 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
 
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==OBJECTIVES==
* Begin fluorescence detection through pressure testing analysis of microsphere samples (new modifications & better results)
* Continue to run DSC sample analysis on microsphere samples previously prepared on '''3/29/13'''
* Continue to remove excess oil from microsphere samples prepared on '''03/29/13'''
* Wash microspheres prepared on '''03/29/13''' with hexanes
* Dry microsphere samples rinsed with hexanes in preparation for DSC and X-ray
* Make films containing both clay and PVOH variations to run X-ray analysis
==Straight Pipette: Microsphere Pressure Samples Tested==
'''RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13'''
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Order'''
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
| align="center" style="background:#f0f0f0;"|'''PVOH Type'''
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
| align="center" style="background:#f0f0f0;"|'''Amount of Hydrogel Used (g)'''
|-
| 1||50:50||146K||110% CEC Laponite w/ DMHXLBR||0.1883
|-
| 2||90:10||146K||NaMT||0.1742
|-
| 3||90:10||130K||110% CEC Laponite w/ DMHXLBR||0.1404
|-
| 4||90:10||146K||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.1721
|-
| 5||50:50||146K||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.1884
|-
| 6||50:50||146K||NaMT||0.1349
|}
==PVOH Film Preparations==
==PVOH Film Preparations==
'''Prepared PVOH films in water:'''
'''RECALL FILM MAKING PROTOCOL ON 9/02/12'''
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)  
 
'''(Actual Mass = 0.4934g)'''
{| {{table}}
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
'''(Actual Mass = 0.9965g)'''
| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
|-
| NONE||NONE||0.49990||NONE||8.0
|-
| 50:50||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.25280||0.2468||8.0
|-
| 90:10||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.45770||0.05030||8.0
|-
| 50:50||110% CEC NaMT w/ DMHXLBR||0.25020||0.25010||8.0
|-
| 90:10||110% CEC NaMT w/ DMHXLBR||0.45020||0.05240||8.0
|}


'''Standard PVOH Film Protocol:'''
==Notes==
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.


'''Notes:'''
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
* Heat was reduced when solutions began to boil too rapidly
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)




Revision as of 22:34, 14 April 2013

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OBJECTIVES

  • Begin fluorescence detection through pressure testing analysis of microsphere samples (new modifications & better results)
  • Continue to run DSC sample analysis on microsphere samples previously prepared on 3/29/13
  • Continue to remove excess oil from microsphere samples prepared on 03/29/13
  • Wash microspheres prepared on 03/29/13 with hexanes
  • Dry microsphere samples rinsed with hexanes in preparation for DSC and X-ray
  • Make films containing both clay and PVOH variations to run X-ray analysis

Straight Pipette: Microsphere Pressure Samples Tested

RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13

Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g)
1 50:50 146K 110% CEC Laponite w/ DMHXLBR 0.1883
2 90:10 146K NaMT 0.1742
3 90:10 130K 110% CEC Laponite w/ DMHXLBR 0.1404
4 90:10 146K 50% CEC NaMT w/ Bu3HdP+ 0.1721
5 50:50 146K 50% CEC NaMT w/ Bu3HdP+ 0.1884
6 50:50 146K NaMT 0.1349

PVOH Film Preparations

RECALL FILM MAKING PROTOCOL ON 9/02/12

PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL)
NONE NONE 0.49990 NONE 8.0
50:50 50% CEC NaMT w/ Bu3HdP+ 0.25280 0.2468 8.0
90:10 50% CEC NaMT w/ Bu3HdP+ 0.45770 0.05030 8.0
50:50 110% CEC NaMT w/ DMHXLBR 0.25020 0.25010 8.0
90:10 110% CEC NaMT w/ DMHXLBR 0.45020 0.05240 8.0

Notes


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