OBJECTIVES
- Begin fluorescence detection through pressure testing analysis of microsphere samples (new modifications & better results)
- Continue to run DSC sample analysis on microsphere samples previously prepared on 3/29/13
- Continue to remove excess oil from microsphere samples prepared on 03/29/13
- Wash microspheres prepared on 03/29/13 with hexanes
- Dry microsphere samples rinsed with hexanes in preparation for DSC and X-ray
- Make films containing both clay and PVOH variations to run X-ray analysis
Straight Pipette: Microsphere Pressure Samples Tested
RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13
Sample Order
|
PVOH vs. Clay Ratio
|
PVOH Type
|
Clay Selection
|
Amount of Hydrogel Used (g)
|
1 |
50:50 |
146K |
110% CEC Laponite w/ DMHXLBR |
0.1883
|
2 |
90:10 |
146K |
NaMT |
0.1742
|
3 |
90:10 |
146K |
110% CEC Laponite w/ DMHXLBR |
0.1404
|
4 |
90:10 |
146K |
50% CEC NaMT w/ Bu3HdP+ |
0.1721
|
5 |
50:50 |
146K |
50% CEC NaMT w/ Bu3HdP+ |
0.1884
|
6 |
50:50 |
146K |
NaMT |
0.1349
|
- Specific parameters used to analyze fluorescence of hydrogel samples containing Rhodamine 6G dye included the following:
# Excitation: 480nm
# Emission Range: 500-650nm
# Excitation Slit Width: 10
# Emission Slit Width: 10
# Scan Speed: 1200
PVOH Film Preparations
RECALL FILM MAKING PROTOCOL ON 9/02/12
PVOH vs. Clay Ratio
|
Clay Selection
|
PVOH 146K Mass (g)
|
Actual Clay Mass (g)
|
H2O Added (mL)
|
NONE |
NONE |
0.49990 |
NONE |
8.0
|
50:50 |
50% CEC NaMT w/ Bu3HdP+ |
0.25280 |
0.2468 |
8.0
|
90:10 |
50% CEC NaMT w/ Bu3HdP+ |
0.45770 |
0.05030 |
8.0
|
50:50 |
110% CEC NaMT w/ DMHXLBR |
0.25020 |
0.25010 |
8.0
|
90:10 |
110% CEC NaMT w/ DMHXLBR |
0.45020 |
0.05240 |
8.0
|
Notes
- On 04/05/13, the DSC instrument lost contact or communication with the computer; therefore, microsphere samples originally prepared on 04/03/13 were incorrectly run and analyzed.
- Therefore, all microsphere samples placed in the DSC on 04/03/13 were re-run over the weekend -- no results could be collected.
|