User:Karmella Haynes/Notebook/BioBrick cloning/2015/08/25: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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* Re-grow cultures for clones with failed sequencing runs
* Re-grow cultures for clones with failed sequencing runs
* Note: DNASU sampling from the 96-well block is top to bottom, then left to right
* From DNASU Order 10294
** Note: DNASU sampling from the 96-well block is top to bottom, then left to right
** A1 = L34-001, A2 = L34-002, A3 = L34-003, etc.
** A1 = L34-001, A2 = L34-002, A3 = L34-003, etc.
* Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling
* Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling
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# L34-027, C4, 399
# L34-027, C4, 399
# L34-032, H4, 339
# L34-032, H4, 339
# L34-040, H5, 494
# L34-050, B7, 303
# L34-055, G7, 563
# L34-071, G9, 237
# L34-075, C10, 300
# L34-078, F10, 647
# L34-080, G10, 495
# L34-082, B11, 308
# L34-084, D11, 498
# L34-093, E12, 8
# L34-094, F12, 611
# L34-096, H12, 814




Latest revision as of 01:05, 27 September 2017

Karmella's BioBrick Cloning Main project page
Previous entry      Next entry

08/25/15

  • Cultures - CRISPR library 5 mL cultures


CRISPR library 5 mL cultures

  • Re-grow cultures for clones with failed sequencing runs
  • From DNASU Order 10294
    • Note: DNASU sampling from the 96-well block is top to bottom, then left to right
    • A1 = L34-001, A2 = L34-002, A3 = L34-003, etc.
  • Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling
  • Scrape bacteria from the original spot agar plates; grow as 5 mL culture for miniprep (overnight)

Clone, well (agar spot position), phred score

  1. L34-003, C1, 313
  2. L34-015, G2, 281
  3. L34-027, C4, 399
  4. L34-032, H4, 339
  5. L34-040, H5, 494
  6. L34-050, B7, 303
  7. L34-055, G7, 563
  8. L34-071, G9, 237
  9. L34-075, C10, 300
  10. L34-078, F10, 647
  11. L34-080, G10, 495
  12. L34-082, B11, 308
  13. L34-084, D11, 498
  14. L34-093, E12, 8
  15. L34-094, F12, 611
  16. L34-096, H12, 814


RESULTS (08/26/15)

  • Only four clones grew
  1. L34-032
  2. L34-040
  3. L34-094
  4. L34-096



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