User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18: Difference between revisions
From OpenWetWare
| Line 10: | Line 10: | ||
==12/18/12== | ==12/18/12== | ||
<!-- Precede finished items with a checkmark ✓ --> | <!-- Precede finished items with a checkmark ✓ --> | ||
* | * TRIzol-RNeasy prep: lyse cells in TriZOL; store at -80°C | ||
---- | ---- | ||
''' | '''TRIzol-RNeasy prep'''<br> | ||
# "K562 1": 1:2 dilution cells, +DNA | # "K562 1": 1:2 dilution cells, +DNA | ||
# "K562 1": 1:2 dilution cells, no DNA | # "K562 1": 1:2 dilution cells, no DNA | ||
| Line 24: | Line 24: | ||
* Pellet cells at r.t./ 3 min./ 2000 rpm | * Pellet cells at r.t./ 3 min./ 2000 rpm | ||
* Discard supernatant | * Discard supernatant | ||
* Resuspend pellet in 500 μL | * Resuspend pellet in 500 μL TRIzol | ||
* Store at -80°C for next steps (Carly) | * Store at -80°C for next steps (Carly) | ||
Revision as of 15:06, 18 December 2012
 Pc-TF Genomics
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
12/18/12
TRIzol-RNeasy prep
| |
