User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18

Pc-TF Genomics

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12/18/12 TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C TRIzol-RNeasy prep "K562 1": 1:2 dilution cells, +DNA "K562 1": 1:2 dilution cells, no DNA "K562 1": 1:4 dilution cells, +DNA "K562 1": 1:4 dilution cells, no DNA "K562 1": 1:8 dilution cells, +DNA "K562 1": 1:8 dilution cells, no DNA Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (did this 4x for each culture); discard remainder of culture Pellet cells at r.t./ 5 min./ 3000 rpm Discard supernatant Resuspend pellet in 500 μL TRIzol Store at -80°C for next steps (Carly)