User:Karmella Haynes/Notebook/Polycomb project/Entry Base: Difference between revisions

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{| class="wikitable" border="0"
{| class="wikitable" border="0"
|-
|-
| '''Wells'''    
| '''Wells'''
| '''Construct'''    
| '''Construct'''
| '''DNA'''        
| '''DNA'''
| '''Volumes'''        
| '''Volumes'''
| '''Fugene'''    
| '''Fugene'''
| '''Opti-MEM'''
| '''Opti-MEM'''
|-
|-
| 1-6
| 1-6  
| KAH59/VNpc
| KAH59/VNpc  
| 25-800 ng
| 25-800 ng
| 0.3, 0.5, 1.0, 2.0, 4.0, 8.0
| 0.3, 0.5, 1.0, 2.0, 4.0, 8.0  
| 1.8 ul
| 1.8 ul  
| 18.2 ul
| 18.2 ul  
|-
|-
| 7-12
| 7-12
| KAH60/VNpc
| KAH60/VNpc
| "
| "
| "
| "
|-
| 13-18
| KAH54/VNpc
| "
| "
| "
| "
|-
| 19-24
| KAH66/MV5
| "
| "
| "
| "
|-
| 25-30
| KAH65/MV5
| "
| "
| "
| "
|-
| 31-36
| KAH59/MV5
| "
| "
| "
| "
|-
| 37-42
| KAH36
| "
| "
| "
| "
|-
| 43-48
| none
| "
| "
| "
| "

Revision as of 07:49, 19 November 2009

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page

11/19/09

  • Transfections: test Gal4/mCh/VP64 ATF's (trial 2)


Transfections
> HEK293 cells + Gal4/mCh/VP64 constructs
> Use Fugene, 24-well format
> Dilute each plasmid to 100 ng/ul in st dH2O


Wells Construct DNA Volumes Fugene Opti-MEM
1-6   KAH59/VNpc   25-800 ng 0.3, 0.5, 1.0, 2.0, 4.0, 8.0   1.8 ul   18.2 ul  
7-12 KAH60/VNpc " " " "
13-18 KAH54/VNpc " " " "
19-24 KAH66/MV5 " " " "
25-30 KAH65/MV5 " " " "
31-36 KAH59/MV5 " " " "
37-42 KAH36 " " " "
43-48 none " " " "



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