User:Karmella Haynes/Notebook/Polycomb project/Entry Base

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Project name Main project page

mm/dd/yy

  • Outline of experiments/ tasks

Task 1
> Line item 1

Reagent Volume Conc.
reagent 1 vol 1 conc 1
reagent 2 vol 2 conc 2
dH2O vol 3  
  total vol   --> Reaction conditions

Lipo transfection
> Details

Wells Plasmid DNA Volume Lipo Opti-MEM (total)
1 name 2 μg ### μL 4 μL 500 μL
2 name " ### μL " "
3 name " ### μL " "
4 name " ### μL " "
5 name " ### μL " "
6 name " ### μL " "

> Add Lipo to 250 μL Opti-MEM --> R.T/ 5 min.
> Add DNA to 250 μL Opti-MEM
> Add DNA mix to Lipo mix --> R.T./ 20 min.
> Remove 500 μL medium from each well; Add 500 μL complexes to each well (4 ml med. each); Grow cells at 37°C
> Refresh medium after 5 hours (ab-free)


Fugene transfection
> Details

Wells Plasmid DNA Volume Fugene Opti-MEM
1-6 name 200 ng ### μL 1.8 μL 18.2 μL
7-12 name " ### μL " "
13-18 name " ### μL " "
19-24 name " ### μL " "

> Master mixes (4, x6): 10.8 μL Fugene + 109.2 μL Opti-MEM --> R.T/ 5 min.
> Add 6x vol DNA --> R.T./ 20 min.
> Add 20 μL complexes to each well (1 ml med. each); Grow cells at 37°C overnight


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