User:Karmella Haynes/Notebook/Polycomb project/Entry Base: Difference between revisions
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> Dilute each plasmid to 100 ng/ul in st dH2O<br> | > Dilute each plasmid to 100 ng/ul in st dH2O<br> | ||
{| class="wikitable" border="0" cellspacing="3" | |||
{| class="wikitable" border="0" | |||
|- | |- | ||
| '''Wells''' | | '''Wells''' | ||
Line 25: | Line 24: | ||
| '''Opti-MEM''' | | '''Opti-MEM''' | ||
|- | |- | ||
| 1-6 | | 1-6 | ||
| KAH59/VNpc | | KAH59/VNpc | ||
| 25-800 ng | | 25-800 ng | ||
| 0.3, 0.5, 1.0, 2.0, 4.0, 8.0 | | 0.3, 0.5, 1.0, 2.0, 4.0, 8.0 | ||
| 1.8 ul | | 1.8 ul | ||
| 18.2 ul | | 18.2 ul | ||
|- | |- | ||
| 7-12 | | 7-12 | ||
Line 81: | Line 80: | ||
| " | | " | ||
|} | |} | ||
> Fugene + Opti-MEM --> R.T/ 5 min. <br> | |||
> Add DNA --> R.T./ 20 min. <br> | |||
> Add complexes to each well (1 ml med. each); 37 <br> | |||
> Assay RFP/ Luc after 18-24 hours | |||
Revision as of 07:53, 19 November 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/19/09
> Fugene + Opti-MEM --> R.T/ 5 min.
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