User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/07: Difference between revisions
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==Bench work== | ==Bench work== | ||
# [[Muratore:Protocols/PCR/QuikChange| QuikChange]] | # [[Muratore:Protocols/PCR/QuikChange#Basic QuikChange protocol| QuikChange]] | ||
#* template = 10 μg/mL [[pTXB1]] | #* template = 10 μg/mL [[pTXB1]] | ||
#* forward primer = 12.5 ng/μL [[Muratore:Materials/Primers#ins_His_after_CBD| ins-His-after-CBD]] | #* forward primer = 12.5 ng/μL [[Muratore:Materials/Primers#ins_His_after_CBD| ins-His-after-CBD]] | ||
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#* anneal step = 7 min | #* anneal step = 7 min | ||
#* 18 melt/anneal/extend cycles | #* 18 melt/anneal/extend cycles | ||
#* [[User:Kathryn Muratore|Kathryn Muratore]] 12:07, 9 June 2011 (EDT): I had the wrong volume of 10X buffer in the original protocol. Therefore, these reactions were run with 2x buffer, not 1x buffer. | |||
# analytical minigel | # analytical minigel | ||
#* 1.2% agarose gel | #* 1.2% agarose gel | ||
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#*→ 1h @ 37°C | #*→ 1h @ 37°C | ||
#*→ store at 4°C | #*→ store at 4°C | ||
==Results== | |||
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Revision as of 09:07, 9 June 2011
 AU CHEM-570 Lab Prep
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Objective
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