User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/24: Difference between revisions
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# Gel purification did not work | # Gel purification did not work | ||
#* Also, note that there is a lot of ethanol in these solutions. | #* Also, note that there is a lot of ethanol in these solutions. | ||
## ''Solution'' - check [DNA] by UV (1:10 dilution should give A<sub>260</sub>=0.05-0.1 according to gel estimates. | ## ''Solution'' - check [DNA] by UV (1:10 dilution should give A<sub>260</sub>=0.05-0.1 according to gel estimates based on mass, but will not be visible based on moles. | ||
##* Do this today! | ##* Do this today! | ||
#* Recall that I did not incubate the melted agarose sample in the column before applying a vacuum. | #* Recall that I did not incubate the melted agarose sample in the column before applying a vacuum. |
Revision as of 12:35, 24 June 2011
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Bench work
Conclusions and future plansPossible problems:
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