User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/12: Difference between revisions

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#* send miniprep samples for DNA sequencing: pTXB1His+BSA colony #1 and pTXB1+BSA colony #2
#* send miniprep samples for DNA sequencing: pTXB1His+BSA colony #1 and pTXB1+BSA colony #2
#* Use T7 promoter and terminator universal primers
#* Use T7 promoter and terminator universal primers
==Results==
* [DNA]:


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Revision as of 10:27, 13 July 2011

AU CHEM-570 Lab Prep <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Bench work

  1. Miniprep (done with Daniel Catt)
    • Follow instructions for miniprep
    • measure concentration of DNA
    • store @ -20°C
    • Forgot to make glycerol stocks
      • after spinning cells, but before re-suspending them, streaked a plate with samples of each cell pellet.
      • → 37°C ~6h
  2. Glycerol stock cultures (done by Daniel Catt)
    1. 3 mL LB + 3μL 100 mg/mL Amp + V(His)+I colony #1 cells from plate in step 1 above
    2. 3 mL LB + 3μL 100 mg/mL Amp + V(His)+I colony #2 cells from plate in step 1 above
    3. 3 mL LB + 3μL 100 mg/mL Amp + V(His)+I colony #3 cells from plate in step 1 above
    4. 3 mL LB + 3μL 100 mg/mL Amp + V+I colony #1 cells from plate in step 1 above
    5. 3 mL LB + 3μL 100 mg/mL Amp + V+I colony #2 cells from plate in step 1 above
    6. 3 mL LB + 3μL 100 mg/mL Amp + V+I colony #3 cells from plate in step 1 above
    • → 37°C w/ shaking O/N
  3. Sequencing
    • send miniprep samples for DNA sequencing: pTXB1His+BSA colony #1 and pTXB1+BSA colony #2
    • Use T7 promoter and terminator universal primers

Results

  • [DNA]:
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