User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/13: Difference between revisions
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==Objective== | ==Objective== | ||
Start making competent cells of expression strain, [[ER2566]]. We're using a new protocol containing hexamminecobalt chloride in the transformation buffer and adding DTT in the process. See Hanahan, D. (1983) JMB '''166''' 557-580. | |||
==Bench work== | ==Bench work== | ||
# | # Glycerol stocks (done by [[User:Daniel Catt|Daniel Catt]]) | ||
#*... | |||
# Competent cell prep | |||
## innoculate 250 mL [[Muratore:Materials/Media/SOB| SOB]] with 6 colonies from ''E. coli'' ER2566 plate from [[../../06/22| a few weeks ago]] | |||
#*→ 18°C w/ shaking | |||
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Revision as of 10:27, 13 July 2011
 AU CHEM-570 Lab Prep
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ObjectiveStart making competent cells of expression strain, ER2566. We're using a new protocol containing hexamminecobalt chloride in the transformation buffer and adding DTT in the process. See Hanahan, D. (1983) JMB 166 557-580. Bench work
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