User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/04/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Experimental Biological Chemistry II</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Purpose== | ||
* | * The purpose of the experiment today is to make starter culture in order to prepare for a 15-hour cell growth experiment tomorrow. | ||
==Procedure== | |||
* 3mL of starter culture, each in 4 falcon tubes were made. | |||
* In 4 falcon tubes labeled "DH5α-T1 starter culture", 3mL of autoclaved LB were pipetted, respectively. | |||
* With a wooden stick, a portion of E.coli cells were picked from cell plates made on [[User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/12|02/12/2013]] and dipped in one falcon tube containing 3mL of autoclaved LB. | |||
* The above procedure was repeated three more times. | |||
* Cultures were placed in shaker at 37°C, 230rpm for 2 hours. | |||
* After shaking, culture were placed in centrifuge at 4°C, 4700rpm, for 30 minutes. | |||
* Supernatants were discarded, and pellet were stored in -80°C until next day morning. | |||
Revision as of 10:53, 2 April 2013
Experimental Biological Chemistry II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Purpose
Procedure
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