User:Khyra A. Neal/Notebook/Chem 571/2014/10/01: Difference between revisions
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* Measure Ca<sup>2+</sup> of dialyzed solutions that contain Ca<sup>2+</sup> ion using ISE | * Measure Ca<sup>2+</sup> of dialyzed solutions that contain Ca<sup>2+</sup> ion using ISE | ||
** Lysozyme dialyzed in both 50 mM CaCl<sub>2</sub> and 500 μM CaCl2 (a total of 4 solutions) | ** Lysozyme dialyzed in both 50 mM CaCl<sub>2</sub> and 500 μM CaCl2 (a total of 4 solutions) | ||
| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Substance''' | | align="center" style="background:#f0f0f0;"|'''Substance''' | ||
| align="center" style="background:#f0f0f0;"|'''Potential (mV)''' | | align="center" style="background:#f0f0f0;"|'''Potential (mV)''' | ||
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* Measure fluorescence from 300 nm-550 nm and excitation at 280 nm | * Measure fluorescence from 300 nm-550 nm and excitation at 280 nm | ||
* Clean fluorescence cuvette after each use with 1 N HCl, methanol and HPLC water | * Clean fluorescence cuvette after each use with 1 N HCl, methanol and HPLC water | ||
Revision as of 19:14, 1 October 2014
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October 1, 2014Analysis of Dialysis SolutionsBradford Analysis
Fluorescence
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