User:Linh N Le/Notebook/2009/08/11: Difference between revisions
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*Since it is already set to max (60) i will leave it there and see if the water gets too hot | *Since it is already set to max (60) i will leave it there and see if the water gets too hot | ||
*Its been ~1hr and the incubator is set to 60. The water in the beaker (which i placed on the far side away from the fan) is reading 40-41C. Thats too hot, so i reduced the incubator to 55 and changed out the water. | *Its been ~1hr and the incubator is set to 60. The water in the beaker (which i placed on the far side away from the fan) is reading 40-41C. Thats too hot, so i reduced the incubator to 55 and changed out the water. | ||
*The incubator set at 55 yielded water at 39 | |||
**Trying setting of 50 | |||
*50 yields 35C water | |||
**Trying 52.5 | |||
*A note is that i have the beaker furthest from the fan. The booklet says that there is a temperature gradient, so close to the fan=hotter | |||
*At 52.5, on the far side of the chamber, the water reads 37c! | |||
==Motility== | ==Motility== | ||
*Andy mentioned yesterday that we may do some microscope stuff | *Andy mentioned yesterday that we may do some microscope stuff | ||
*He may want to try his lipids on some microtubes | *He may want to try his lipids on some microtubes | ||
*He spin coated a small cover glass with the lipds | |||
**Half of the slide was covered with tape | |||
*We put MT's on the slide and looked at it under the scope | |||
**We saw MT's stuck to the glass but not to the lipids (as wanted) | |||
*We used the old kinesin Andy drummed up from yesterday, but with no luck | |||
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Revision as of 16:59, 11 August 2009
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To Do
Incubator
Motility
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