User:Linh N Le/Notebook/2009/08/26: Difference between revisions
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*My notes say that we resuspended the tubulin on [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/06/15 6/15/2009] | *My notes say that we resuspended the tubulin on [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/06/15 6/15/2009] | ||
*[http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/06/15/TRITC_%28Rhodamine%29_and_regular_tubulin Koch's notes] here as well | *[http://openwetware.org/wiki/User:Steven_J._Koch/Notebook/Kochlab/2009/06/15/TRITC_%28Rhodamine%29_and_regular_tubulin Koch's notes] here as well | ||
*[http://www.openwetware.org/wiki/User:Brigette_D._Black/Notebook/Brigettes_Notebook/2009/06/15/Making_Tubulin Brigette's Notes] | |||
**We made both Rhodamine and unlabeled tubulin | **We made both Rhodamine and unlabeled tubulin | ||
*Koch also have [http://openwetware.org/index.php?title=Koch_Lab:Protocols/Kinesin/Tubulin_resuspension_and_polymerization&oldid=307098#Non-labeled_tubulin_resuspension a great page] about resuspending tubulin and polymerizing it | *Koch also have [http://openwetware.org/index.php?title=Koch_Lab:Protocols/Kinesin/Tubulin_resuspension_and_polymerization&oldid=307098#Non-labeled_tubulin_resuspension a great page] about resuspending tubulin and polymerizing it | ||
==Procedure== | ==Procedure== | ||
''Note: It is important to keep the tubulin cold, so prepare all the tubes, buffers and LN2 before you start'' | ''Note: It is important to keep the tubulin cold, so prepare all the tubes, buffers and LN2 before you start'' |
Revision as of 13:38, 26 August 2009
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To Do
Tubulin
ProcedureNote: It is important to keep the tubulin cold, so prepare all the tubes, buffers and LN2 before you start
Rhodamine Tubulin
Unlabeled Tubulin
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