User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/04: Difference between revisions

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==September 4,2013==
==September 4, 2013==
===Objective===
===Objective===
Execute the following [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/04 procedure] examining protein fluoresecence.
Continue procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-571_2013F/2013/09/03 yesterday].
===Procedure===
*Selected protein:
*Stock solution:
*Dilutions:
**10μM
**5μM
**1μM
**0.5μM


===Data===
===Data===
====Absorbance Measurements====
====Absorption Spectra====
[[Image:2013_0904_inosine_spectra.PNG]]<br.>
All peaks at 249nm. <br.>


====Fluorescence Measurements====
===Calculations and Analysis===
 
Error analysis table:<br.>
===Analysis===
[[Image:2013_0904_error_analysis_table.PNG]]<br.>
====Integration====
Separated by concentration; the average, standard deviation, and outliers (via Q-test) were found for the entire classes' data.
 
====Calibration Curves====
====Normalization=====
<u>Group Data</u><br.>
 
[[Image:2013_0904_inosine_calibration_curve.PNG]]<br.>
 
<u>Class Data</u><br.>
 
[[Image:2013_0904_Group_Data.PNG]]
[[Category:]]
=====Determining Unknown's Concentration=====
[[Category:Miscellaneous]]
*Unknown from: Zach, Eleni, Moira
*Concentration determined from our calibration curve: 1.164x10<sup>-5</sup>
*Concentration determined from class calibration curve: 1.323x10<sup>-5</sup>
*Actual concentration:


[[User:Matt Hartings|Matt Hartings]] I'd like to see the group calibration curve. I'd also like some quantification of error in your concentration calibrations.




Revision as of 19:04, 11 September 2013

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September 4, 2013

Objective

Continue procedure from yesterday.

Data

Absorption Spectra

<br.> All peaks at 249nm. <br.>

Calculations and Analysis

Error analysis table:<br.> <br.> Separated by concentration; the average, standard deviation, and outliers (via Q-test) were found for the entire classes' data.

Calibration Curves

Group Data<br.> <br.> Class Data<br.>

Determining Unknown's Concentration
  • Unknown from: Zach, Eleni, Moira
  • Concentration determined from our calibration curve: 1.164x10-5
  • Concentration determined from class calibration curve: 1.323x10-5
  • Actual concentration:

Matt Hartings I'd like to see the group calibration curve. I'd also like some quantification of error in your concentration calibrations.




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