User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/01: Difference between revisions
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== | ==October 1, 2013== | ||
===Objective=== | ===Objective=== | ||
#UV-Vis Absorbance of reactants, catalysts, and products | |||
#*Make 10mL stock solution of luminol at appropriate dilution | |||
#Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer | |||
===Description=== | ===Description=== | ||
<u>UV-vis Samples</u><br.> | |||
*Blank: TRIS buffer | |||
*Horseradish peroxidase | |||
*H<sub>2</sub>O<sub>2</sub> | |||
*20x luminol | |||
*10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H<sub>2</sub>O<sub>2</sub> catalyzed by 333uL HRP) | |||
===Data=== | ===Data=== | ||
<u>Absorbance Measurements</u><br.> | |||
[[Image:2013_1001_absorbance.PNG]]<br.> | |||
<u>Beer's Law concentration calculations</u><br.> | |||
[[Image:2013_1001_beer's_law_calcs.PNG]]<br.> | |||
<u>Kinetic Measurements</u> | |||
===Notes=== | |||
Revision as of 09:13, 2 October 2013
 Experimental Biological Chemistry: Fall 2013
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October 1, 2013Objective
DescriptionUV-vis Samples<br.>
DataAbsorbance Measurements<br.>
Kinetic Measurements
Notes | |
