User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/02: Difference between revisions
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===Objective=== | ===Objective=== | ||
<u>Chemiluminescence</u> | |||
*Tube 1 (10mL): 0.5mM luminol, 5nM HRP | *Tube 1 (10mL): 0.5mM luminol, 5nM HRP | ||
*Tube 2 (10mL): 5mM H<sub>2</sub>O<sub>2</sub> (from stock 12.8mM) | *Tube 2 (10mL): 5mM H<sub>2</sub>O<sub>2</sub> (from stock 12.8mM) | ||
<u>Redo Kinetics</u> | |||
*Determine appropriate concentrations of reagents (HRP needs to be 10000x less concentrated than luminol, to slow reaction rate) | |||
*Double concentrations to increase absorbance, to monitor bigger changes | |||
===Description=== | ===Description=== | ||
Revision as of 09:25, 2 October 2013
 Experimental Biological Chemistry: Fall 2013
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October 2, 2013ObjectiveChemiluminescence
Redo Kinetics
DescriptionDataNotes | |
