User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/11/12

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(November 12, 2013)
Current revision (17:12, 12 November 2013) (view source)
 
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*Each sample diluted from Au stock in the above manner
*Each sample diluted from Au stock in the above manner
===Data===
===Data===
-
<u>Atomic Absorption</u>
+
<u>Atomic Absorption</u><br.>
-
 
+
[[Image:2013_1112_AA_gold_standards.PNG]]
-
 
+
[[Image:2013_1112_AA_lysozyme.PNG]]
-
<u>UV-vis Absoprtion</u>
+
[[Image:2013_1112_AA_hemoglobin.PNG]]
 +
[[Image:2013_1112_AA_HRP.PNG]]
 +
[[Image:2013_1112_AA_pepsin.PNG]]
===Notes===
===Notes===
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+
*UV-vis measurements to be taken tomorrow

Current revision

Experimental Biological Chemistry: Fall 2013 Main project page
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November 12, 2013

Objective

  1. Atomic Absorption of Lysozyme and Hemoglobin nanoparticles synthesized 11/06/2013
  2. UV-vis measurements of Lysozyme and Hemoglobin nanoparticles synthesized 11/06/2013

Description

Atomic Absorption:

  • Centrifuge 4mL from each sample for 30min at 4000rpm
  • Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
  • Take measurements of your standards and samples
  • Make a graph showing concentration of Au vs Au:protein ratio
Gold Standards- Calculations
  1. 50 ug/mL:
    • (1000ug/mL)V=(50ug/mL)(5mL)
    • V= 0.25mL Au stock in 4.75mL water
  • Each sample diluted from Au stock in the above manner

Data

Atomic Absorption
Image:2013_1112_AA_gold_standards.PNG Image:2013_1112_AA_lysozyme.PNG Image:2013_1112_AA_hemoglobin.PNG Image:2013_1112_AA_HRP.PNG Image:2013_1112_AA_pepsin.PNG

Notes

  • UV-vis measurements to be taken tomorrow





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