User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/04: Difference between revisions

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**Final [Au]=0.5mM, 5mL total volume
**Final [Au]=0.5mM, 5mL total volume
[[Image:2014_0204_Au_Myo_NPs_cheat_sheet.PNG]]
[[Image:2014_0204_Au_Myo_NPs_cheat_sheet.PNG]]
===Procedure===
===Procedure===
*Make twelve gold:myoglobin (100:1) nanofiber solutions with a final Au concentration of 0.5mM and final volume of 5mL
*Make twelve gold:myoglobin (100:1) nanofiber solutions with a final Au concentration of 0.5mM and final volume of 5mL

Revision as of 11:28, 11 February 2014

Nanofiber>Scaffold Procedure 1 Results <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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February 4, 2014

Objectives

  • If nanofibers formed in the tall scaffolds by the original nanofiber procedure (from 01/29/2014):
    • Take pictures against white background
    • Print taller scaffolds that span the length of liquid in the test tube
    • Increase the concentrations of gold and myoglobin, keeping the ratio the same

Nanofiber Results

100:1 Gold:Myoglobin Nanofibers alone<br.> <br.> 100:1 Gold:Myoglobin Nanofibers on PLA scaffolds<br.> <br.> 100:1 Gold:Myoglobin Nanofbiers on PLA sacffold- under microscope<br.>

Note

Because the nanofibers turned out black instead of purple, we will double the concentration of both gold and myoglobin in the initial solution, keeping the 100 to 1 ratio the same.

Sample Preparation

  • Au stock solution:
    • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
  • Myoglobin stock solution:
    • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
  • Volumes of Au, Myoglobin, and water for each test tube:
    • Final [Au]=0.5mM, 5mL total volume

Procedure

  • Make twelve gold:myoglobin (100:1) nanofiber solutions with a final Au concentration of 0.5mM and final volume of 5mL
    • One of these solutions will have one of the extra tall scaffolds in solution
    • One of these solutions will have four of the tall scaffolds in solution
    • One of these solutions will have one of the tall scaffolds in solution
    • The other nine solutions will be made as usual to make nanofibers for the "brute force" method
  • All solutions will go into the oven at 80C for four hours to allow nanofibers to form



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