February 4, 2014
Objectives
- If nanofibers formed in the tall scaffolds by the original nanofiber procedure (from 01/29/2014):
- Take pictures against white background
- Print taller scaffolds that span the length of liquid in the test tube
- Increase the concentrations of gold and myoglobin, keeping the ratio the same
Nanofiber Results
100:1 Gold:Myoglobin Nanofibers alone<br.>
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100:1 Gold:Myoglobin Nanofibers on PLA scaffolds<br.>
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100:1 Gold:Myoglobin Nanofbiers on PLA sacffold- under microscope<br.>
Note
Because the nanofibers turned out black instead of purple, we will double the concentration of both gold and myoglobin in the initial solution, keeping the 100 to 1 ratio the same.
Sample Preparation
- Au stock solution:
- 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
- Myoglobin stock solution:
- 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
- Volumes of Au, Myoglobin, and water for each test tube:
- Final [Au]=0.5mM, 5mL total volume
Procedure
- Make twelve gold:myoglobin (100:1) nanofiber solutions with a final Au concentration of 0.5mM and final volume of 5mL
- One of these solutions will have one of the extra tall scaffolds in solution
- One of these solutions will have four of the tall scaffolds in solution
- One of these solutions will have one of the tall scaffolds in solution
- The other nine solutions will be made as usual to make nanofibers for the "brute force" method
- All solutions will go into the oven at 80C for four hours to allow nanofibers to form
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