User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/12: Difference between revisions
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==June 12, 2013== | ==June 12, 2013== | ||
=== | *Finished MHA silica-coated AuNPs, resuspended in water, 10x and 100x dilutions made for all four samples | ||
*Take absorbance spectra of sonicated original | *Fluorescent beads and Alexa 532 antibodies successfully run and analyzed on FCS | ||
*Take absorbance spectra of 10x and 100x | **Green fluorescent beads, diluted 10000x | ||
**Alexa 532 antibodies, diluted to 500pM and 100pM | |||
*Take fluorescence spectra of | ===Notes=== | ||
* | *Take absorbance spectra of sonicated original AuNP dilutions | ||
*Take absorbance spectra of 10x and 100x silica-coated AuNP dilutions | |||
*Take fluorescence spectra of ZnPPIX/AuNP, DNA/ThT combinations | |||
*Add ZnPPIX/AuNP fluorescence data to graph for presentation | |||
*Make DNA/ThT calibration curve (with fluorescence data) | |||
Revision as of 13:17, 12 June 2013
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June 12, 2013
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