User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/08/29: Difference between revisions
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==Preparation of Gold and Bovine Serum Albumin Stock Solutions== | ==Preparation of Gold and Bovine Serum Albumin Stock Solutions== | ||
* Gold (Au) stock solution was made by dissolving .085 g of hydrogen tetrachloroaurate (HAuCl<sub>4</sub>) in .025 L of water to obtain 10 mM. | * Bovine Serum Albumin (BSA) was prepared by dissolving .0249 g of white crystalline powder of BSA into .025 L of water to obtain 15 μM of BSA. | ||
* Gold (Au) stock solution was made by dissolving .085 g of hydrogen tetrachloroaurate (HAuCl<sub>4</sub>) in .025 L of water to obtain 10 mM HAuCl<sub>4</sub>. | |||
* The amount of .085 g was acquired from the following calculation: | * The amount of .085 g was acquired from the following calculation: | ||
.025 L of water × (10×10<sup>-3</sup> mM of HAuCl<sub>4</sub> ÷ 1L)= .00025 mM of HAuCl<sub>4</sub> | .025 L of water × (10×10<sup>-3</sup> mM of HAuCl<sub>4</sub> ÷ 1L)= .00025 mM of HAuCl<sub>4</sub> | ||
.00025 mM of HAuCl<sub>4</sub> × 339. 79 g (formula weight of HAuCl<sub>4</sub>)= .085 g of HAuCl<sub>4</sub> | .00025 mM of HAuCl<sub>4</sub> × 339. 79 g (formula weight of HAuCl<sub>4</sub>)= .085 g of HAuCl<sub>4</sub> | ||
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Preparation of Gold and Bovine Serum Albumin Stock Solutions
.025 L of water × (10×10-3 mM of HAuCl4 ÷ 1L)= .00025 mM of HAuCl4 .00025 mM of HAuCl4 × 339. 79 g (formula weight of HAuCl4)= .085 g of HAuCl4
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