User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/04: Difference between revisions
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==UV-Vis Spectroscopy of Aug. 29 Au/BSA solutions== | ==UV-Vis Spectroscopy of Aug. 29 Au/BSA solutions== | ||
* In reference to the entry of Wednesday, August 29 (a week ago), the Au and BSA solutions were made using a metal spatula. Due to its hygroscopic nature, HAuCl<sub>4</sub> reacted with the metal spatula. | * In reference to the entry of Wednesday, August 29 (a week ago), the Au and BSA solutions were made using a metal spatula. Due to its hygroscopic nature, HAuCl<sub>4</sub> reacted with the metal spatula. | ||
* The Au/BSA solutions of Aug. 29 were still examined using UV-Vis. The values for each mole ratio were plotted by its intensity vs. time using MS Excel. | * The Au/BSA solutions of Aug. 29 were still examined using UV-Vis. The values for each mole ratio were plotted by its intensity vs. time using MS Excel. | ||
* Micropipets were used to transfer the Au/BSA solution from their test tube to the cuvette. | |||
* One single cuvette was used for all UV-Vis scans to avoid fluctuations in the UV-Vis scan readings. | |||
[[Image:AuBSAaug29edited.png]] | [[Image:AuBSAaug29edited.png]] |
Revision as of 20:39, 14 September 2012
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UV-Vis Spectroscopy of Aug. 29 Au/BSA solutions
Preparation of Au and BSA solutions
0.254 g of BSA × (1 mol of BSA ÷ 66430 g of BSA) = 3.82 × 10-7 3.82 × 10-7 ÷ .025 L of water = 1.53 × 10-5 mM/L
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