User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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==Filtration of Buffers== | ==Filtration of Buffers== | ||
[[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration]] | [[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration]] | ||
* The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. | * The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the flask was connected to a Welch vacuum pump. | ||
* | |||
* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen randomly. | |||
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Revision as of 02:17, 5 October 2012
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Lysis of transformed E. coli
Filtration of Buffers
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