User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution). | * The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution). | ||
* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle. | * The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle. | ||
* The bottles were capped with aluminum foil tops and refrigerated. | * The bottles were capped with aluminum foil tops and refrigerated. The buffers were clear, colorless liquid solution before and after filtration. | ||
==Filtration of the Supernatant== | ==Filtration of the Supernatant== |
Revision as of 02:35, 5 October 2012
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Lysis of transformed E. coli
Filtration of Buffers
Filtration of the Supernatant |