User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/26: Difference between revisions
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* On the dialog box, pump > gradient > B > 100%. | * On the dialog box, pump > gradient > B > 100%. | ||
* Once the peak levels back to baseline, pump > gradient > B > 0%. This washes the system with A. | * Once the peak levels back to baseline, pump > gradient > B > 0%. This washes the system with A. | ||
* At Flowpath > injection valve > position > inject. Switch to position 1. This bypasses the | * At Flowpath > injection valve > position > inject. Switch to position 1. This bypasses the column with the superloop's binding buffer flow at at 10 mL/min on inject. Then press end. | ||
* Sample 1 was loaded onto the super loop with the syringe at inject. The position was switched at position 3. The flow rate was at 5 mL/min with the gradient of B at 0%. From inject, the setting was switch to load. | |||
* The desired baseline should reach an absorbance of 280 and 250. Then hit pause. | |||
* The fraction is under the Frac 900 function in the dialog box. 5 mL fraction size was chosen. Then hit continue. | |||
* On the pump function, the load was set at 0% B after 25 mL the flow rate of 0 mL/min. was changed to 5 mL/min. | |||
* The same steps were done for sample 2. | |||
[[Image:ADA HisTrapE.PNG]] | [[Image:ADA HisTrapE.PNG]] |
Revision as of 10:05, 5 October 2012
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Purification of Proteins
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