User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/09: Difference between revisions
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* The reaction of 3-aminophthalhydrazide (luminol) and hydrogen peroxide is catalyzed by the addition of horseradish peroxidase (HRP) producing 3-aminophthalate. | * The reaction of 3-aminophthalhydrazide (luminol) and hydrogen peroxide is catalyzed by the addition of horseradish peroxidase (HRP) producing 3-aminophthalate. | ||
* The reagents originated from the HRP Assay of aminoantipyrine (AAP) laboratory period [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02|10/02/2012.]] For this procedure, 4-iodophenol, hydrogen peroxide, HRP, and water will be used. The AAP will be replaced in the reaction with luminol. | * The reagents originated from the HRP Assay of aminoantipyrine (AAP) laboratory period [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02|10/02/2012.]] For this procedure, 4-iodophenol, hydrogen peroxide, HRP, and water will be used. The AAP will be replaced in the reaction with luminol. | ||
* It was decided to prepare 10 mM of luminol since this will be further diluted down to 3 mM upon the addition of water in the cuvette. | |||
* The following calculations were made for luminol (FW 177.16): | |||
Revision as of 18:27, 25 October 2012
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Atomic Absorption Spectroscopy of Au/BSA
Chemiluminescence of Luminol
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