User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/17: Difference between revisions
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* When the solution is clear, the agarose was poured into the gel electrophoresis tray with the comb in place. | * When the solution is clear, the agarose was poured into the gel electrophoresis tray with the comb in place. | ||
* A wait period of 30 min. was allotted for the gel to solidify. Upon observing the gel is already in its solidified state, the comb was gently removed to avoid breakage. | * A wait period of 30 min. was allotted for the gel to solidify. Upon observing the gel is already in its solidified state, the comb was gently removed to avoid breakage. | ||
* | * The chamber containing the gel tray was filled with TAE buffer to the point where the gel is completely submerged of the buffer. | ||
Revision as of 09:26, 27 October 2012
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Electrophoresis of the mutated DNA plasmids
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