User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/13: Difference between revisions
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* By manipulating Beer's Law, the molar absorptivity was calculated from the absorbance and concentration of the substances. | * By manipulating Beer's Law, the molar absorptivity was calculated from the absorbance and concentration of the substances. | ||
<math>\frac{\epsilon}{ | <math>\frac{\epsilon A}{bc}</math> | ||
Revision as of 08:53, 9 December 2012
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ADA Kinetic Assay Preparations
V1 = 0.08934 mL = 89.3 μL in 5 mL of buffer
UV-visible scans of Reagents
M1V1 = M2V2 (3 mM)(10 μL) = M2 (1000 μL) M2 = .0307 mM
3 μL of ADA × 65 μM = M2 (1000 μL) M2 = .195 μM of ADA
M2 = .0975 μM = 97.5 nM
Second dilution M = 48.8 nM Final Concentration = 24.38 nM
Beer's Law
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