User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/11/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Dialysis Results== | ||
* | * Dialysis tubes were prepared for adenosine deaminase (ADA) fractions 2 and 8 on 11/14/12. The ADA were removed from the dialysis tubes. | ||
* ADA fraction 2 appeared clear and colorless with no signs of precipitation. | |||
* ADA fraction 8 precipitated out of solution. | |||
==Kinetics of ADA== | |||
* The activity of ADA was measured by monitoring the absorbance of inosine over time. | |||
[[Image:AbsoofIIno.png]] | |||
* The trends among runs 1, 2, and 3 seem consistent. The increase of the concentration of the enzyme with each addition increases the rate of production of inosine with respect to time. | |||
NOTE: The remaining period was used to edit formatting for Class Literature Survey | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|''Volume of ADA (μL)'' | |||
| align="center" style="background:#f0f0f0;"|''Concentration of ADA (μM)'' | |||
| align="center" style="background:#f0f0f0;"|''Volume of phosphate buffer (mL)'' | |||
| align="center" style="background:#f0f0f0;"|''Concentration of phosphate buffer (M)'' | |||
| align="center" style="background:#f0f0f0;"|''Volume of adenosine (μL)'' | |||
| align="center" style="background:#f0f0f0;"|''Concentration of adenosine (mM)'' | |||
|- | |||
| 15||0.21||2.95||0.0492||50||0.0512 | |||
|- | |||
| 25||0.35||2.95||0.0492||50||0.0512 | |||
|- | |||
| 40||0.56||2.95||0.0492||50||0.0512 | |||
|- | |||
| 50||0.70||2.95||0.0492||50||0.0512 | |||
|- | |||
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Latest revision as of 22:17, 26 September 2017
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Dialysis Results
Kinetics of ADA
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