User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/28

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Objective

Cassidy and Sarah got strange results for their fluorescence measurements yesterday (myoglobin, AuNPs, pH4). So I'm going to re run their samples.

Description

  1. Add experimental record here. Include what, how, and why...

Data

Stock solution for class

  1. AgNO3
    1. 28.13 mg AgNO3
    2. to 10.0 mL in water
    3. 169.87 g/mol
    4. 16.6 mM

Myoglobin Sample Prep

  1. 250 μM AuCl3, 12.5 μM myoglobin, pH 4
    1. 176 μL 4.27 mM AuCl3
    2. 234 μL 160 μM myoglobin
    3. 300 μL 1mM HCl
    4. 2290 μL water


UV-Vis of sample before and after measurement

Fluorescence data

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Notes

Anneliese Faustino




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