User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/29

Biomaterials Design Lab

Main project page Previous entry      Next entry

Objective Run fluorescence scans for BSA, AuNP, pH 6, 80C Lysozyme, pH 5, 80C BSA AuNP Fluorescence 3 mL Sample 0.25 mM AuCl3 Stock concentration 4.27 mM Volume Added: 176 μL 3.125 μM BSA Stock Concentration 52.6 μM Volume Added: 178 μL pH 6 Stock Concentration 1 mM HCl Volume Added: 3 μL Volume water added: 2643 μL UV-Vis of sample Fluorescence scans Acquisition information Excitation: 290 nm, 10 nm slit width Emission: 310-540 nm, 10 nm slit width 100 nm/minute 61 measurements, 180 second delay between measurements Lysozyme Fluorescence 3 mL Sample 0.5 μM Lysozyme Stock Concentration 210 μM Volume Added: 7.1 μL pH 5 Stock Concentration 1 mM HCl Volume Added: 30 μL Volume Water Added: 2963 μL UV-Vis of sample Fluorescence scans Acquisition information Excitation: 290 nm, 10 nm slit width Emission: 310-540 nm, 10 nm slit width 100 nm/minute 61 measurements, 180 second delay between measurements Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.