User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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[[Image:20160930 mrh LysozymepH6 scans.png]] | [[Image:20160930 mrh LysozymepH6 scans.png]] | ||
==Myoglobin Fluorescence== | |||
<u>3 mL Sample</u> | |||
# 12.5 μM BSA | |||
## Stock Concentration 160 μM | |||
## Volume Added: 234 μL | |||
# pH 5 | |||
## Stock Concentration 1 mM HCl | |||
## Volume Added: 30 μL | |||
# Volume water added: 2736 μL | |||
<u>UV-Vis of sample</u> | |||
[[Image:20160930 mrh Myoglobin UVVis.png]] | |||
Evidence of protein aggregation (cloudy solution) | |||
<u>Fluorescence scans</u> | |||
# Acquisition information | |||
## Excitation: 290 nm, 10 nm slit width | |||
## Emission: 310-540 nm, 10 nm slit width | |||
## 100 nm/minute | |||
## 61 measurements, 180 second delay between measurements | |||
[[Image:20160930 mrh MyoglobinpH5 IntegratedI.png]] | |||
[[Image:20160930 mrh MyoglobinpH5 EmissionMax.png]] | |||
[[Image:20160930 mrh MyoglobinpH5 scans.png]] | |||
==Data== | ==Data== |
Latest revision as of 02:03, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
ObjectiveAcquire fluorescence scans of lysozyme (pH 6) and myoglobin (pH 5) at 80C. Lysozyme Fluorescence3 mL Sample
UV-Vis of sample Evidence here of photobleaching of the fluorophore Fluorescence scans
Myoglobin Fluorescence3 mL Sample
UV-Vis of sample Evidence of protein aggregation (cloudy solution) Fluorescence scans
Data
NotesThis area is for any observations or conclusions that you would like to note.
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