# User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/01/29

(Difference between revisions)
 Revision as of 01:05, 7 February 2014 (view source) (→Entry title)← Previous diff Revision as of 01:06, 7 February 2014 (view source) (→Entry title)Next diff → Line 6: Line 6: | colspan="2"| | colspan="2"| - ==Entry title== + ==Method== * There were a total of 12 gold: nanofiber solutions made, with all of them being the same * There were a total of 12 gold: nanofiber solutions made, with all of them being the same *The ratio of myoglobin to gold was 100:1 and the concentration of gold was 0.25mM *The ratio of myoglobin to gold was 100:1 and the concentration of gold was 0.25mM Line 12: Line 12: *The other 9 will be used to make the other two methods *The other 9 will be used to make the other two methods *They were put in an oven for 4hrs at 80°C *They were put in an oven for 4hrs at 80°C + ==Calculations== ==Calculations== *Au stock solution: *Au stock solution:

## Revision as of 01:06, 7 February 2014

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## Method

• There were a total of 12 gold: nanofiber solutions made, with all of them being the same
• The ratio of myoglobin to gold was 100:1 and the concentration of gold was 0.25mM
• Three of the solutions will have the scaffolds in them as this is our first method
• The other 9 will be used to make the other two methods
• They were put in an oven for 4hrs at 80°C

## Calculations

• Au stock solution:
• 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
• Myoglobin stock solution:
• 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
• Volumes of Au, Myoglobin, and water for each test tube:
• Final [Au]=0.25mM, 5mL total volume