User:Megan L. Channell/Notebook/Horseradish/2013/09/10: Difference between revisions
From OpenWetWare
Line 11: | Line 11: | ||
==Protocol== | ==Protocol== | ||
# Make a stock solution with 10.4 mg HRP in | # Make a stock solution with 10.4 mg HRP in 2 mL of Tris buffer. | ||
# Actual caluclulated concnetration 5200μg/mL | # Actual caluclulated concnetration 5200μg/mL | ||
# Standards were made, 1.9 μg/mL, 2.2 μg/mL, 2.7 μg/mL, 3 μg/mL, 3.3 μg/mL, 7 μg/mL | # Standards were made, 1.9 μg/mL, 2.2 μg/mL, 2.7 μg/mL, 3 μg/mL, 3.3 μg/mL, 7 μg/mL | ||
Line 21: | Line 21: | ||
# Make two blanks with 800μL buffer and 200 μL Assay reagent | # Make two blanks with 800μL buffer and 200 μL Assay reagent | ||
# Repeat the entire process for a total of two trials | # Repeat the entire process for a total of two trials | ||
*Tris Buffer iwas 50.02mM Tris and 50.52mM NaCl | |||
Gold AA/ICPMS Standards | Gold AA/ICPMS Standards | ||
Line 30: | Line 31: | ||
*A 10 mL volumetric flask was used to make these standards | *A 10 mL volumetric flask was used to make these standards | ||
*Note water is used to make solutions | *Note water is used to make solutions | ||
*Stock Solution 1000±10μg/mL | |||
==Data== | ==Data== |
Revision as of 11:27, 18 September 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Horseradish Peroxidase UV-Vis and Gold SolutionObjectiveDetermine Horseradish Peroxidase (HRP) concentration through a calibration curve. This is done through the Bradford Assay. Protocol
Gold AA/ICPMS Standards
Data
NoteTrial 1 and 2 were done with a cuvette that had a path length of .412 cm. Trial 1 and 2 were not accurate when looking at the calibration curve. A new set of standards will be made for tomorrow. |