User:Melvin Colorado Escobar/Notebook/chem 472/2016/03/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
*Take UV Vis data of the 24 hour samples of 25% red nanoparticles and 25% purple nanoparticles, made with DI water, that were set up last [[User:Jamie Nunziata/Notebook/Biomaterial Design 2016/2016/02/24|Wednesday]]. | |||
*Take UV Vis data of stock solutions after a serial dilution of both the lysozyme nanoparticles, made with DI water, and the purple nanoparticles, made with spring water, made last [[User:Jamie Nunziata/Notebook/Biomaterial Design 2016/2016/02/24|Wednesday]] | |||
*Create lysozyme nanoparticles with spring water instead | |||
*Set up plants in 25% lysozyme nanoparticles, made with DI water, and 25% purple nanoparticles, made with spring water. | |||
==Protocol== | |||
<b>UV Vis of Stock Solutions</b> | |||
<br>Four serial dilutions (half dilution) were made of the lysozyme nanoparticles, synthesized on 02/22/2016, and were run on UV-Vis. The same dilutions were performed using the purple nanoparticles made with spring water. | |||
<br><br> | |||
<b>NP Uptake Setup</b> | |||
<br>25% solutions of both the lysozyme nanoparticles with normal water and purple AuNPs with spring water (synthesized on 02/22/2016). Ferns with similarly extensive root systems were added to the solutions and were analyzed after 2 hours and 24 hours via UV Vis. | |||
<br><br> | |||
<b>Lysozyme Nanoparticle Setup</b><br> | |||
100mL stock solution was made using the following steps: | |||
#15.21mg of lysozyme was added to 25mL of water in a volumetric flask (42.55mM). The solutions was mixed and added to the beaker. | |||
#0.84mL of 1% by weight HAuCl4 stock solution was added (made on [[User:Jamie_Nunziata/Notebook/Biomaterial_Design_2016/2016/01/27|02/23/2016]]) | |||
#74.16mL of water was added, bringing the final solution volume up to 100mL. | |||
The solution was then covered with foil and placed in the oven for 4 hours at 80 degrees celsius. | |||
<br><br> | |||
==Results== | |||
UV-Vis data was taken of the 25% solutions red and purple citrate gold nanoparticles, made with regular water, that set up for uptake with java ferns for 24 hours last week. | |||
<br><br> | |||
<b>Figure 1</b>: UV-Vis spectra for 25% Red Citrate AuNPs | |||
<br>[[Image:JMB_RedAuNP_UVVis_final.png]] | |||
<br><br><br> | |||
<b>Figure 2</b>: UV-Vis spectra for Lysozyme AuNPs in Spring Water | |||
<br>[[Image:JMB_LysozymeAuNP_UVVis_final.png]] | |||
<br><br><br> | |||
<b>Figure 3</b>: UV-Vis spectra for 25% Purple Citrate AuNPs | |||
<br>[[Image:JMB_PurpleAuNP_UVVis_final.png]] | |||
<br><br><br> | |||
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Latest revision as of 01:46, 27 September 2017
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Objective
ProtocolUV Vis of Stock Solutions
The solution was then covered with foil and placed in the oven for 4 hours at 80 degrees celsius.
ResultsUV-Vis data was taken of the 25% solutions red and purple citrate gold nanoparticles, made with regular water, that set up for uptake with java ferns for 24 hours last week.
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