User:Meng Xiao He/Notebook/fall08/2008/12/10: Difference between revisions

From OpenWetWare
< User:Meng Xiao He‎ | Notebook‎ | fall08‎ | 2008‎ | 12
Jump to navigationJump to search
Line 19: Line 19:


==Plating on sucrose==
==Plating on sucrose==
*1:4 dilution into fresh LB, let grow, then plate
*1:2 dilution into fresh salt-free-LB, let grow, then plate 100ul
*40ul ferric citrate added to plates for B colonies
*40ul ferric citrate added to plates for B colonies


<!-- end -->
<!-- end -->
|}
|}

Revision as of 15:37, 10 December 2008

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html> Constants
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PCR tests

  • use 12-8 protocol on cco brown colonies 1 and 2: each takes cco5F, cco3R, and just pair of 3 outers
  • use Platinum adaptation of 12-8 protocol:
Cycling conditions:
  1. 5 min at 94
  2. 30s at 94
  3. 30s @ 61, 57 for 40 cycles
  4. 7min @ 72 (should capture WT cells as well), only 2min for last 12 cycles
  5. 10 min@ 72 to end
  6. hold at 4

Plating on sucrose

  • 1:2 dilution into fresh salt-free-LB, let grow, then plate 100ul
  • 40ul ferric citrate added to plates for B colonies
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Meng_Xiao_He/Notebook/fall08/2008/12/10&oldid=269213"