User:Meng Xiao He/Notebook/fall08/2008/12/11: Difference between revisions
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lanes 1-6 (Phusion, 12-8 program): | lanes 1-6 (Phusion, 12-8 program): | ||
*cco12.9B outers+cco5F, outers+cco3R, outers+water | *cco12.9B outers+cco5F, outers+cco3R, outers+water | ||
** this particular cell is WT, but does not show the band seen [[User:Meng_Xiao_He/Notebook/fall08/2008/12/09#12.2F7_Platinum_PCR_results| here]]? | |||
*cco12.9C outers+cco5F, outers+cco3R, outers+water | *cco12.9C outers+cco5F, outers+cco3R, outers+water | ||
**Confused... why are there 2 bands? Unless it's actually a KO??? | |||
* 1kb, 100 bp ladders | * 1kb, 100 bp ladders | ||
* Platinum (annealing temp of 61) cco12.9D outers+cco5F, outers+cco3R, outers+water | * Platinum (annealing temp of 61) cco12.9D outers+cco5F, outers+cco3R, outers+water |
Revision as of 10:54, 11 December 2008
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PCRlanes 1-6 (Phusion, 12-8 program):
Platinum protocols seems not to work... Replating
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