User:Meng Xiao He/Notebook/fall08/2008/12/12

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(Replating on Gm, sucrose)
(Replating on Gm, sucrose)
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==Replating on Gm, sucrose==
==Replating on Gm, sucrose==
* colonies replated on both sucrose and Gm
* colonies replated on both sucrose and Gm
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* 5 cbb and 6 cco colonies Phusion PCRed using 12-8 program> note that flanking pairs were only the ko gene and Duo as the control (no orientation primer)
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* 5 cbb and 6 cco colonies Phusion PCRed using PhFLANK program (12-8 modified to have 3:45 extension) > note that flanking pairs were only the ko gene and Duo as the control (no orientation primer)
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Revision as of 12:29, 12 December 2008

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Replating on Gm, sucrose

  • colonies replated on both sucrose and Gm
  • 5 cbb and 6 cco colonies Phusion PCRed using PhFLANK program (12-8 modified to have 3:45 extension) > note that flanking pairs were only the ko gene and Duo as the control (no orientation primer)
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