User:Michael F. Nagle/Notebook/Chem 571/2012/08/29: Difference between revisions

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## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
## 3.75*10^-7mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] * 66,463g/mol [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] = .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]]
## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution
## .0249g [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] was put in 25mL to make a 15μM solution
while good, this needs more description. what are the numbers that you are listing? theoretical concentration? actual mass added? actual concentration? etc.
==Results==
==Results==
*The BSA solution created was 15μM
*The BSA solution created was 15μM

Revision as of 11:03, 17 September 2012

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Objective

  • Abigail E. Miller 14:02, 17 September 2012 (EDT):didn't you also make various ratio solutions foHAuCl4 to BSA to synthesize AuNPs?

Procedure

  1. A stock solution of HAuCl4 was made, with the attempted Molarity of 10mM.
    1. (moles HAuCl4)/.025L H2O = .01M
    2. 2.5*10-4 mol HAuCl4
    3. 2.5*10-4 mol * 339.785mol/g HAuCl4 = .0849g
    4. .0197g of the .0849g stuck to the weigh paper, leaving .0656gHAuCl4.
    5. .0656g HAuCl4 * (1molHAuCl4/339.79g/molHAuCl4)= xM HAuCl4
    6. .007721M HAuCl4
    7. .0849g HAuCl4 was put in 25mL to make a 10mM solution
  2. A 15μM stock solution of BSA was made
    1. (moles BSA)/.025L = .000015
    2. 3.75*10^-7mol BSA
    3. 3.75*10^-7mol BSA * 66,463g/mol BSA = .0249g BSA
    4. .0249g BSA was put in 25mL to make a 15μM solution

while good, this needs more description. what are the numbers that you are listing? theoretical concentration? actual mass added? actual concentration? etc.

Results

  • The BSA solution created was 15μM
  • The actual molarity for the HAuCl4 stock solution created turned out to be 7.721mM rather than 10mM. This is because unlike BSA, which is easy to handle, HAuCl4 comes in large chunks which don't break easily and melts, leaving residue on the weigh paper.


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