User:Michael S. Bible/Notebook/571/2014/08/27: Difference between revisions

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==Objective==
==Colloid v. Fiber Formation==
Learn how to maintain an OpenWetWare Notebook.
*Creating a 125 μM stock solution of Lysozyme
**50 mL Lysozyme solution, (1.25x10<sup>-4</sup> M)(0.050 L)(14300 g/mol)
***0.0894g Lysozyme in 50 mL Volumetric Flask
*Creating a 2.512 mM stock solution of HAuCl4
**50 mL HAuCl4 solution, (2.5x10<sup>-3</sup> M)(0.050 L)(340 g/mol)
***0.043 g HAuCl4 in 50 mL Volumetric Flask


==Description==
Six solutions of differing [Au]:[Protein] ratios were made with ratios from 10:1 to 60:1. 0.5 mL of HAuCl4 was always used and differing amounts of protein.  Water was added to make each solution volume 5 mL. See chart below for volumes of solutions used. The test tubes in which the solutions were combined were then placed in an oven a 80°C for 3 hours.
# Add experimental record here. Include what, how, and why...


==Data==
* Add data and results here...


==Notes==
This area is for any observations or conclusions that you would like to note.


==Kinetics of AuNP Formation==
Enough Lysozyme was added to three test tubes each containing 0.5 mL HAuCl<sub>4</sub> to make [Au]:[Lysozyme]=30 (see chart below for prescription to make such a solution).  The test tubes were covered with foil and placed in an 80°C hot water bath.  Every 30 minutes half of the solution in one of the test tubes was poured into a plastic cuvette and placed in the UV-Vis Spectrometer.  The UV-Vis from 800 nm to 360 nm was recorded and the above procedure was performed every 30 minutes until no more solution remained.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
==<font color=#0f1028></font>==
 
{|style="width:700px"
[[Category:Course]]
|<u>HAuCl<sub>4</sub></u>
[[Category:Miscellaneous]]
|<u>Lysozyme</u>
 
|<u>H<sub>2</sub>O</u>
 
|-
 
|0.5 mL
[[Category:Course]]
|1.0 mL
[[Category:Miscellaneous]]
|3.5 mL
|-
|0.5 mL
|0.5 mL
|1.0 mL
|-
|0.5 mL
|0.33 mL
|4.17 mL
|-
|0.5 mL
|0.25 mL
|4.25 mL
|-
|0.5 mL
|0.20 mL
|4.30 mL
|-
|0.5 mL
|0.167 mL
|4.33 mL
|-


<br>




Revision as of 17:46, 1 September 2014

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Colloid v. Fiber Formation

  • Creating a 125 μM stock solution of Lysozyme
    • 50 mL Lysozyme solution, (1.25x10-4 M)(0.050 L)(14300 g/mol)
      • 0.0894g Lysozyme in 50 mL Volumetric Flask
  • Creating a 2.512 mM stock solution of HAuCl4
    • 50 mL HAuCl4 solution, (2.5x10-3 M)(0.050 L)(340 g/mol)
      • 0.043 g HAuCl4 in 50 mL Volumetric Flask

Six solutions of differing [Au]:[Protein] ratios were made with ratios from 10:1 to 60:1. 0.5 mL of HAuCl4 was always used and differing amounts of protein. Water was added to make each solution volume 5 mL. See chart below for volumes of solutions used. The test tubes in which the solutions were combined were then placed in an oven a 80°C for 3 hours.


Kinetics of AuNP Formation

Enough Lysozyme was added to three test tubes each containing 0.5 mL HAuCl4 to make [Au]:[Lysozyme]=30 (see chart below for prescription to make such a solution). The test tubes were covered with foil and placed in an 80°C hot water bath. Every 30 minutes half of the solution in one of the test tubes was poured into a plastic cuvette and placed in the UV-Vis Spectrometer. The UV-Vis from 800 nm to 360 nm was recorded and the above procedure was performed every 30 minutes until no more solution remained.


HAuCl4 Lysozyme H2O
0.5 mL 1.0 mL 3.5 mL
0.5 mL 0.5 mL 1.0 mL
0.5 mL 0.33 mL 4.17 mL
0.5 mL 0.25 mL 4.25 mL
0.5 mL 0.20 mL 4.30 mL
0.5 mL 0.167 mL 4.33 mL


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