User:Michael S. Bible/Notebook/571/2014/08/27

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Colloid v. Fiber Formation

  • Creating a 125 μM stock solution of Lysozyme
    • 50 mL Lysozyme solution, (1.25x10-4 M)(0.050 L)(14300 g/mol)
      • 0.0894g Lysozyme in 50 mL Volumetric Flask
  • Creating a 2.512 mM stock solution of HAuCl4
    • 50 mL HAuCl4 solution, (2.5x10-3 M)(0.050 L)(340 g/mol)
      • 0.043 g HAuCl4 in 50 mL Volumetric Flask

Six solutions of differing [Au]:[Protein] ratios were made with ratios from 10:1 to 60:1. 0.5 mL of HAuCl4 was always used and differing amounts of protein. Water was added to make each solution volume 5 mL. See chart below for volumes of solutions used. The test tubes in which the solutions were combined were then placed in an oven a 80°C for 3 hours.


Data

  • Add data and results here...


HAuCl4 Lysozyme H2O
0.5 mL 1.0 mL 3.5 mL
0.5 mL 0.5 mL 1.0 mL
0.5 mL 0.33 mL 4.17 mL
0.5 mL 0.25 mL 4.25 mL
0.5 mL 0.20 mL 4.30 mL
0.5 mL 0.167 mL 4.33 mL


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