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Objective
To use BSA (bovine serum albumin) to synthesize gold nanoparticles. BSA reduces Au3+ to Au 0 for the synthesis to occur.
Description
- Add 0.0015 mM BSA and 0.25 mM of HAuCl4 to 10 mL of 50mM acetate buffer (pH 5.46) at room temperature. The ratio of Au to BSA is 167 for optimum results.
- Note: distilled water used to rinse gold and BSA into mixture from weigh boats.
- Four-place balance used to determine weights. Actual weights determined to be 0.0011g BSA and 0.0004g gold.
- Heat mixture at 80°C for four hours
- Using a UV-Vis Spectrophotometer, obtain spectra every 30 minutes, starting at t=0 minutes
- Calculations:
- Au
- 0.25mM Au = 0.00025 mol / L = 2.5 x 10-7 mol / mL
- 2.5 x 10-7 mol / mL = x / 10 mL
- x = 2.5 x 10-6 mol / mL x 196.967 g/mol = 4.92 x 10-4g
- BSA
- 0.0015mM BSA = 0.0000015 mol / L = 1.5 x 10-9 mol / mL
- 1.5 x 10-9 mol / mL = x / 10 mL
- x = 1.5 x 10-9 mol / mL x 66776 g/mol = 1.00 x 10-3g
Data
- Add data and results here...
Notes
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Project name
