User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/03/07: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Au/BSA Mole Ratios</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
*To analyze various ratios of solution made on [[User:Michaela_Harper/Notebook/Biological_Chemistry_Lab/2012/03/06|03/06/2012]]
*To determine the effect of the mole ratio of Au/BSA on AuNP synthesis by refining the range in which the greatest change occurs.


==Description==
==Description==
# Add experimental record here. Include what, how, and why...
 
Effect of Au/BSA Ratio
 
# Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl<sub>4</sub>:
#* 132 Au/BSA
#* 134 Au/BSA
#* 136 Au/BSA
#* 138 Au/BSA
#* 140 Au/BSA
#* 142 Au/BSA
#* 144 Au/BSA
#* 146 Au/BSA
#* 148 Au/BSA
# Dilute to 1 mL with water.
# Place in oven at 80°C.
# Leave solutions in oven for 3 hr.
# Centrifuge solutions at 13200 rpm for 10 min.
# Take UV-vis data of supernatants.


==Data==
==Data==


The following graphs show the effect of varying ratios in between the purple nanoparticle solution ratio (70) and the fiber solution ratio (166).  
The following graphs show the effect of varying ratios in between the purple nanoparticle solution ratio (70) and the fiber solution ratio (166). This data is from solutions made on [[User:Michaela_Harper/Notebook/Biological_Chemistry_Lab/2012/03/06|03/06/2012]].


[[Image:Varying_ratios.png|500px]]
[[Image:Varying_ratios.png|500px]]
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==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.
The data from solutions with ratios varying between the purple solution and fiber solution (between 70 and 166) showed a dramatic decrease between 130 and 150 Au/BSA, indicating a switch to more fiber formation. This led us to test more ratios in between 130 and 150, with increments of 2 to refine the range where the change occurs.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Miscellaneous]]
 


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Revision as of 11:43, 7 March 2012

Au/BSA Mole Ratios <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

  • To analyze various ratios of solution made on 03/06/2012
  • To determine the effect of the mole ratio of Au/BSA on AuNP synthesis by refining the range in which the greatest change occurs.

Description

Effect of Au/BSA Ratio

  1. Make solutions with the following Au/BSA mole ratios using 0.1 mL HAuCl4:
    • 132 Au/BSA
    • 134 Au/BSA
    • 136 Au/BSA
    • 138 Au/BSA
    • 140 Au/BSA
    • 142 Au/BSA
    • 144 Au/BSA
    • 146 Au/BSA
    • 148 Au/BSA
  2. Dilute to 1 mL with water.
  3. Place in oven at 80°C.
  4. Leave solutions in oven for 3 hr.
  5. Centrifuge solutions at 13200 rpm for 10 min.
  6. Take UV-vis data of supernatants.

Data

The following graphs show the effect of varying ratios in between the purple nanoparticle solution ratio (70) and the fiber solution ratio (166). This data is from solutions made on 03/06/2012.



The following graph shows the relationship between the ratio of Au/BSA and the peak absorbance.

Notes

The data from solutions with ratios varying between the purple solution and fiber solution (between 70 and 166) showed a dramatic decrease between 130 and 150 Au/BSA, indicating a switch to more fiber formation. This led us to test more ratios in between 130 and 150, with increments of 2 to refine the range where the change occurs.


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