User:Moira M. Esson/Notebook/CHEM-571/2013/11/19: Difference between revisions

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==AuNP solution preparation==
==AuNP solution preparation==
*The general protocol for the preparation of AuNPs described on [[User  
*The general protocol for the preparation of AuNPs described on [[User  
 
<br>
==AA spectroscopy==
<br>
#Transfer 3mL of sample from test tubes into a 10mL falcon tube.
#Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
#Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]].
#Run samples on AA.




Revision as of 11:50, 19 November 2013

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Objectives

  1. Prepare AuNP-protein solutions for biomineralization
  2. Analyze AuNP-protein solutions prepared on 10/30/2013 using AA spectroscopy.


AuNP solution preparation

  • The general protocol for the preparation of AuNPs described on [[User


AA spectroscopy


  1. Transfer 3mL of sample from test tubes into a 10mL falcon tube.
  2. Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
  3. Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on 09/10/2013.
  4. Run samples on AA.



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