User:Moira M. Esson/Notebook/CHEM-571/2013/11/13

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Objectives

The objective for today is to analyze protein-AuNP solutions prepared on 11/06/2013 using atomic absorption spectroscopy

AA Spectroscopy

  • The general protocol for atomic absorption described on 11/05/2013 was followed. The samples were centrifuged for 30minutes prior to analysis.


Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
100.19.9
200.29.8
300.39.7
400.49.6
500.59.5


Table 2. AA spectroscopy AuNP-protein solutions observations

Au:Lysozyme Ratio Appearance of solution Au:Hemoglobin Ratio Appearance of solution
5Colorless30Lightly colored
10Lightly colored50Lightly colored
15Colored darkly70Darkly colored
20Colored90Darkly colored
25Colored110Colorless
30Colored130Colorless
35Colored150Colorless
40Colorless170Colorless
45Colorless190Colorless
50Colorless210Colorless


  • All of the solutions, if stated as colored, had a purplish color.


Figure 1. Absorbance spectra of AuNP Lysozyme solutions
Image:Atomic Absorbance Spectra of AuNP Lysozyme solutions zem .png
Figure 2. Absorbance Spectra of AuNP-Hemoglobin solutions
Image:Atomic Absorbance Spectra of AuNP-Hemoglobin solutions zem.png
Figure 3. Calibration Curve for Gold Standards
Image:Calibration Curve for Gold Standards lysozymehemozem.png
Figure 4. Concentration vs. Absorbance Au-Hemoglobin solutions Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png
Figure 5. Concentration vs. Absrobance AuNP-Lysozyme solutions
Image:Concentration vs. Absorbance of AuNP-Lysozyme solutions zem.png

Notes

  • The AuNP-protein solutions prepared on 10/30/2013 will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged


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