User:Moira M. Esson/Notebook/CHEM-571/2013/11/19: Difference between revisions
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#Analyze AuNP-protein solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] using AA spectroscopy. | #Analyze AuNP-protein solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] using AA spectroscopy. | ||
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==AuNP solution preparation== | ==AuNP solution preparation== | ||
*The general protocol for the preparation of AuNPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]] was followed. | *The general protocol for the preparation of AuNPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]] was followed. | ||
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#Run samples on AA. | #Run samples on AA. | ||
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Table | Table 6. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution | ||
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| 50||0.5||9.5 | | 50||0.5||9.5 | ||
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Figure 1. Calibration curve of gold standard solutions | |||
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[[Image:Calibration Curve of Gold Standards zem11192013 .png]] | |||
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Figure 2. Atomic Absorbance Spectra of Myoglobin:AuNP solutions | |||
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[[Image:Atomic Absorbance Spectra of Myoglobin AuNP solutions zem11192013.png]] | |||
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Figure 3. Atomic Absorbance Spectra of Lysozyem:AuNP solutions | |||
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[[Image:Atomic Absorbance Spectra of Lysozyme AuNP solutions zem 11192013 .png]] | |||
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*The only solution that was colored was the 30 ratio. All other solutions appeared colorless. | |||
Figure 4. Absorbance vs. Concentration plot of Myoglobin:AuNP solutions | |||
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[[Image:Absorbance vs. Concentration plot of Myoglobin-AuNP solutions zem11192013.png]] | |||
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Figure 5. Absorbance vs. Concentration plot of AuNP-Lysozyme Solutions | |||
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[[Image:Absorbance vs. Concentration plot of Lysozyme-AuNP solutions zem11192013.png]] | |||
Revision as of 11:06, 10 December 2013
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Objectives
AuNP solution preparation
Notes
AA spectroscopy
Figure 4. Absorbance vs. Concentration plot of Myoglobin:AuNP solutions
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