Objectives
- Finish rinsing and filtering prepared microspheres with hexanes.
- Continue microsphere preparation.
- Add dye to prepared microspheres in preparation for diffusion testing on microspheres with dye additive.
Microsphere Dye Addition
- The general protocol for dye addition to PVA hydrogel/microspheres described on 2013/02/08 was followed.
- An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels
- Dye was added to all the microspheres prepared on 2013/02/20
Dilution calculations:
1μM Rhodamine 6G Dye Concentration (90:10)
M1V1 = M2V2
1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL
1μM Rhodamine 6G Dye Concentration (50:50)
M1V1 = M2V2
1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Microsphere preparation
- The general protocol for microsphere preparation described on 2013/02/20 and 2013/02/22.
- The following preparations were run on a 0.1g scale as opposed to a 1g scale in the hopes of preventing the formation of hydrogels.
- A very small amount of PVA/clay was added to each vial and the vial was filled with safflower oil prior to the rapid freezing in order to achieve a true emulsion of microspheres in the organic safflower oil. This may prevent the formation of hydrogels.
PVOH vs. Clay Ratio
|
Clay Selection
|
PVOH 146K Mass (g)
|
Actual Clay Mass (g)
|
H2O Added (mL)
|
Safflower oil Added (mL)
|
50:50 |
110% CEC NaMT |
0.05010 |
0.05100 |
20 |
35
|
90:10 |
110% CEC NaMT |
0.01070 |
0.09100 |
20 |
35
|
Notes
- Dye was added to the microspheres prepared on 2013/02/20 prior to a DSC analysis. These samples will need to be reprepared for DSC analysis.
|